@article {1574694,
	title = {Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells},
	journal = {Cell Stem Cell},
	volume = {27},
	number = {4},
	year = {2020},
	month = {2020 Oct 01},
	pages = {663-678.e8},
	abstract = {Mutant KRAS is a common driver in epithelial cancers. Nevertheless, molecular changes occurring early after activation of oncogenic KRAS in epithelial cells remain poorly understood. We compared transcriptional changes at single-cell resolution after KRAS activation in four sample sets. In addition to patient samples and genetically engineered mouse models, we developed organoid systems from primary mouse and human induced pluripotent stem cell-derived lung epithelial cells to model early-stage lung adenocarcinoma. In all four settings, alveolar epithelial progenitor (AT2) cells expressing oncogenic KRAS had reduced expression of mature lineage identity genes. These findings demonstrate the utility of our in~vitro organoid approaches for uncovering the early consequences of oncogenic KRAS expression. This resource provides an extensive collection of datasets and describes organoid tools to study the transcriptional and proteomic changes that distinguish normal epithelial progenitor cells from early-stage lung cancer, facilitating the search for targets for KRAS-driven tumors.},
	issn = {1875-9777},
	doi = {10.1016/j.stem.2020.07.022},
	author = {Dost, Antonella F M and Moye, Aaron L and Vedaie, Marall and Tran, Linh M and Fung, Eileen and Heinze, Dar and Villacorta-Martin, Carlos and Huang, Jessie and Hekman, Ryan and Kwan, Julian H and Blum, Benjamin C and Louie, Sharon M and Rowbotham, Samuel P and Sainz de Aja, Julio and Piper, Mary E and Bhetariya, Preetida J and Bronson, Roderick T. and Emili, Andrew and Mostoslavsky, Gustavo and Fishbein, Gregory A and Wallace, William D and Krysan, Kostyantyn and Dubinett, Steven M and Yanagawa, Jane and Kotton, Darrell N and Kim, Carla F}
}
